Sepbox 2D-250 system

Sepiatec GmbH develops separation systems based on HPLC (High Performance Liquid Chromatography) and SFC (Supercritical Fluid Chromatography) technology, enabling our customers to speed up their separation and method development significantly.

The Sepbox 2D-250 benchtop model is designed for the automated two-dimensional separation of up to

250 milligrams of extract.

The system offers the choice between liquid and adsorbed (solid) sample injection. Up to 576 fractions can be collected in microtiter plates in just 24 hours.

The Sepbox 2D-250 is suitable for medium to non-polar compounds (Polar Setup optional).

The system is equipped with intuitive, user-friendly software for method development, process control, data acquisition and data analysis.



  • Isolation of compounds with a speed and accuracy unachievable with conventional chromatography methods
    • On average 20 to 30 per cent pure fractions (ELSD) per run
    • Isolation of large quantities possible
    • Reproducible separations
    • Cost advantages due to automation

Purity of Sepbox fractions

On average after one Sepbox run, up to 20 per cent of all fractions from plant extracts are 90 to 99 per cent pure (ELSD*), and from extracts made of marine organisms, up to 30 per cent are 90 to 99 per cent pure (ELSD).

As well as these highly purified compounds, the user will usually find a large number of fractions with compounds whose purity levels are between 50 and 90 per cent in ELSD detection. Results vary depending on the extract and the examined organism.

* The purity levels quoted here were calculated using the 100 per cent method with ELSD detection, as UV detection does not provide reliable purity levels due to different absorption coefficients.

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Modes of operation Medium to non-polar, Polar Setup (optional)
Sample size Max. 250 mg of extract
Pumps 2 HPLC pumps
Columns 1 injection column, 1 main separation column, 3 second separation step, 18 trap columns
Detection UV detector, ELSD detector (optional)
Fraction collector Fraction collector with microtiter plates, 24 deep-well, 48 or 96 well plates
Modes of collection Time and peak based
Valves 13 electrically actuated valves
Software Sepbox Control Software
Dimensions 155 x 62 x 97 cm (W x D x H)