Sepiatec GmbH develops separation systems based on HPLC (High Performance Liquid Chromatography) and SFC (Supercritical Fluid Chromatography) technology, enabling our customers to speed up their separation and method development significantly.
Sepiatec‘s new Prep SFC M5-50 system with XY or carousel fraction collector and 30 ml pump heads for CO2 and modifier pump is a preparative system for separating chiral and achiral samples using supercritical fluid chromatography. The Prep SFC M5 system consists of the main module and the collector module.
Both modules form a functional unit that can be configured in a variety of ways with different equipment.
The main module can be equipped with up to three high pressure pumps, a CO2 pump, a modifier pump and an optional add-on pump for separations with low modifier portions.
The available options include:
- Pumps with 30 ml pump heads, flow rates 0.2 – 30.0 ml/min, 400 bar
- Add-on-pumps with 30 ml pump heads, flow rates 0.1 – 30.0 ml/min, 400 bar
- Versatile SFC system for chiral and achiral separations
- 30 ml pumps for CO2 and organic modifiers
- Column oven for 10 columns with 15 to 30 mm ID
- DAD, ELSD or mass spectrometer optional
- Various fraction collectors available, up to 195 fractions
- Build-in system controller, no additional PC required
- Easy to operate software, stack functions, on-the-fly editing
- Big 15.6” touch screen
The integrated auto sampler can accommodate two standard or deep-well microtiter plates or racks with up to 60 sample vials with a volume of 1.5 ml each.
The column oven contains up to 10 separation columns with internal diameter of 4 to 30 mm and a length of up to 250 mm. The columns are switched using two integrated column switching valves.
The column oven is heatable up to 70° Celsius. Standard equipment in the main module is a UV detector for measuring in the range of 190 – 500 nm. Additional detectors, such as an ELSD, can also be connected.
An optional compact mass spectrometer is available for separations with mass directed fractionation.
An integrated control computer and 15.6“ TFT touch screen is used to input all data for sample separations. No additional computer is required, reducing the space required for the Prep SFC M5 system to a width of just 91.5 cm.
The collector module has a continuously-operating gas-liquid separator that makes a vertical movement when switching to a new fraction vessel.
A valve closes the outlet of the gas-liquid separator while the vessel is being changed.
A new collection simply involves lowering a short capillary into the opening of the fraction vessel, which virtually precludes the risk of carry-over..
The easily exchangeable round holder for the fraction collector vials provides ideal overview.
It is available in several models: 38 vials each of 10 ml volume, 22 vials with 40 ml or 12 flasks with 100 ml volume each. The collector module has a removable and transparent protective cover and comes with a connector port for an aspirator that facilitates use at a standard laboratory bench.
Prep SFC M5 System with Sorter Module
Possibilities for collecting and processing fractions using the Prep SFC M5 system can be expanded with the optional sorter module. The sorter module is a handling robot designed for interacting with the collector module of the Prep SFC M5 system. It is available in a variety of different sizes and can be equipped with various gripper arms. A wide range of variants and functions is possible with the addition of various components:
- Continuous removal of vials from the collector module with sorting according to pre-selected criteria
- Continuous placement of new empty vials into the holder of the collector module, thus expanding the collection capacity to several hundred fractions
- Weighing of vials that are removed from the collector module, which are then to be dried in an evaporator, automatic preparation of a “counter vial” with identical weight, positioning of both vials in the holding tray of the evaporator
- A variety of other sample preparation processes for further analysis of collected fractions
Prep SFC M5 Software
The Prep SFC M5 system is controlled entirely by means of the Prep SFC control software which offers a clear menu structure enabling simple and intuitive operation. All input options are presented on just a few screens. The software includes a sequence editor for inputting separation parameters for different samples,
functions such as stack injection with automatic suggestions for stack times and multiple options for fractionation. A computer at a separate work site can also be connected. If an optional mass spectrometer is used, control and data acquisition also take place directly via the Prep SFC control software.
Technical Specifications
Operating mode |
1 semi-preparative or preparative column |
Solvents |
CO2 and organic solvents |
Number of modifier solvents |
1 standard, max. 4 solvents with optional selection valve |
CO2 pump |
300 ml heads, flow rates 0.1 – 30.0 ml/min, 400 bar, |
Modifier pump |
30 ml heads, flow rates 0.2 – 30.0 ml/min, 400 bar |
Add-on pump, 30 ml (optionally) |
30 ml pump head, flow rates 0.1 – 30.0 ml/min, 400 bar |
Operating pressure |
Up to 300 bar, back pressure regulator online adjustable |
Auto sampler |
2 standard microtiter plates or 2 deep well MTP with 24 x 10 ml, racks 60 vials 1.5 ml each or 24 x 5 ml |
Number of columns and size |
Max. 10 columns, 4 to 30 mm ID, up to 250 mm length |
Column oven |
Ambient temperature to 70°C, integrated flow cell |
UV Detector |
Standard, wavelength 190 to 500 nm |
ELSD |
Optionally available |
Mass spectrometer |
Optionally available |
Fraction collector |
XY or carousel fraction collector |
Software |
Prep SFC control software, Sequence Editor M5 software |
System controller |
Integrated PC, Windows, 15.6” TFT touch-screen |
Connections |
3 USB ports, Ethernet, HDMI |
Dimensions, weight |
91.5/98 (xy collector) x 60 x 88 cm (W x D x H), 148 kg |