Prep SFC 660 System

Sepiatec GmbH develops separation systems based on HPLC (High Performance Liquid Chromatography) and SFC (Supercritical Fluid Chromatography) technology, enabling our customers to speed up their separation and method development significantly.

The Prep SFC 660 system with 3 pumps, developed by Sepiatec, represents a powerful solution for preparative separations by means of supercritical fluid chromatography. The unit has been designed for flow rates of up to 660 ml of supercritical fluid per minute with 400, 250 and 150 ml pump heads, great flow accuracy over the entire range. Depending on the modifier portion, even up to 660 ml/min achieved (in the case of a modifier portion of 40%).

Walk-in laboratory hood

All of the advantages of the SFC technology are combined: Quick separation, a reduced consumption of organic solvents and thus cost, increased safety and an environmentally friendly process.

In spite of its remarkable capacity, the Prep SFC 660 system features a compact design with measurements of 150 x 68 x 178 cm (W x D x H), so that integration within a walk-in laboratory hood does not create any problem.

Additionally, below the unit, several large fraction vessels or a safety cabinet for solvents may be placed. A CO2- as well as a modifier pump are provided, each of them equipped with 400, 250 and 150 ml pump heads and approved for a pressure of 360 bar.

Up to three different organic modifiers can be mixed and used for separations.

Up to 50 °C heatable column oven

Beside the column mounting, the injection valve and the UV flow cell are also integrated within the column oven, which is heatable up to 50 °C. Separation columns with inner diameters between 25 and 50 mm and a length of up to 800 mm are admissible.

When working in “Single Mode“, up to 8 fractions can be collected in “Tandem Mode“ 4 fractions are available. The collection of the fractions is performed in unpressurised state; therefore screening of the vessels for reasons of safety is not necessary.

The fraction volume in the Prep SFC 660 system is not limited.

Fractions of any size may be collected and simply be tapped for further processing.

The equipment of the Prep SFC 660 system includes:

  • a high pressure pump for CO2
    • a high pressure pump for organic modifiers
    • a gradient module for the mixing of modifiers
    • a heatable column oven
    • a back pressure regulator
    • a syringe pump for sample injection
    • a UV detector
    • 8 gas-liquid-separators for fractions
    • a powerful cooling module
    • an integrated computer with touch-screen
    • the Prep SFC 660 control software

The unit is subjected to the control of the Prep SFC control software, which has been developed with maximum user friendliness in mind. Information required, like separation parameters, system setup and fractionating conditions, is selected via touch screen in no time.

Numerous functions

The intuitive menu structure allows entry of the values in consecutively appearing windows, thus enabling also users not permanently involved with these works, to use the software without particular support.

The flexibility is considerably enhanced by numerous functions useful for preparative SFC separations, e.g. stack injection and different modes for collection of fractions.
Before starting a new batch, parameters can be tested and optimized by means of a particular “Manual Screen“.

A powerful computer is integrated within the unit; further space for supplementary electronics is not required. The TFT touch screen is placed in an ergonomically favorable position on the front of the unit. A separate monitor may be connected via HDMI, three USB sockets and a network connection are provided, too.

External cooling device included in main configuration, Simultaneous connection to the pre-cooling and the CO2 pump

Optional equipment for the Prep SFC 660 system:

  • CO2 recycling module for Prep SFC 660 system, For the conversion of gaseous to liquid

  CO2, recovery rate about 85%

  • Compact mass spectrometer, m/z 1200
  • Compact mass spectrometer, m/z 2000

Operating mode 1 preparative column
Suitable columns 30 to 50 mm ID, max. 800 mm in length
Solvents CO2 and organic solvents
Number of modifier solvents 3 solvents, mixable, solvent selection valves included
Total flow rate 660 ml/ min at a modifier portion of 40%
Pump 1, CO2 400 ml pump head, 400 bar
Pump 2, Modifier 250 ml pump head, 400 bar
Pump 3, Modifier/ Add-on 150 ml pump head, 400 bar
Operating pressure Up to 300 bar, back pressure regulator online

adjustable

Injection Partial and total loop with syringe pump
Column oven Ambient temperature to 50° C, 1 column standard, 2 columns with optional valves
UV Detector Wavelength range 190 to 500 nm
Other detectors DAD, ELSD or mass spectrometer optional
Number of fractions Single mode: 1 to 8 fractions

Tandem mode: 1 to 4 fractions

CO2 Recycling With additional CO2 recycling module (optional), gaseous to liquid CO2 conversion, about 85% recovery
Software Prep SFC control software
System controller Integrated controller,
Screen 15.6” touch screen, 16:9 format
Connections 3 USB ports, Ethernet, HDMI
Dimensions 150 x 68 x 178 cm (W x D x H)